English Polski
Akademia Morska w Szczecinie

DSpace Home

DSpace/Manakin Repository

Title
Kinetic properties of Lipase obtained from Pseudomonas aeruginosa isolated from crude oil contaminated soil

ISSN printed: 1733-8670, ISSN on-line: 2392-0378
Femi-Ola, Titilayo Olufunke1; Orjiakor, Paul Ikechukwu2; Enerijiofi, Kingsley Erhons3; Oke, Iyanuoluwa Omolade4; Fatoyinbo, Adebayo Adeola5
URI: https://repository.am.szczecin.pl/handle/123456789/2542
2019-10-04
Soil contaminated with crude oil has negatively affected some of the communities in Ekiti State region in Nigeria. There is an increased interest in microbial lipases because of their enormous potential in industrial and biotechnological applications. This study investigated the characteristics of partially-purified lipase from Pseudomonas aeruginosa isolated from oil-contaminated soil in Ado-Ekiti, Ekiti State. The lipase was purified by gel-filtration, having a molecular weight of 39.11 kDa, with Km and Vmax values of 12.50 and 28.86, respectively. The enzyme had an optimal pH of 8.0 and exhibited its maximal activity at 50°C, whereas the relatively stable temperature and pH were 40°C and 5.0, respectively. The enzyme activity was enhanced by olive oil, which served as the carbon source. Sodium chloride enhanced lipase activity, while calcium chloride acted as mild inhibitor, and iron chloride acted a strong inhibitor. The lipase from Pseudomonas aeruginosa possessed properties of an industrial enzyme and will be useful for biodegradation and bioremediation studies
  1. Ajuzie, C.U., Atuanya, E.I. & Enerijiofi, K.E. (2015) Biodegradation potentials of microorganisms isolated from Eleme Petrochemical industrial effluent. Nigerian Society for Experimental Biology Journal 15 (4), pp. 128–136.,
  2. Amin, M., Bhatti, H.N. & Perveen, F. (2008) Production, partial purification and thermal characterization of Amylase from Fusarium solani in solid-state fermentation. Journals of Chemistry and Biotechnology 30, pp. 480–485.,
  3. Bhatti, H.N. & Nawaz, S. (2009) Production of endoglucanase by Fusarium solani grown in solid-state fermentation. Asian Journal of Chemistry 21 (3), pp. 1943–1948.,
  4. Bhatti, H.N., Rashid, M.H., Nawaz, R., Asgher, M., Perveen, R. & Jabbar, A. (2007) Optimization of media for enhanced glucoamylase production in solid-state fermentation by Fusarium solani. Journals of Food Technology and Biotechnology 45, pp. 51–56.,
  5. Bora, L. & Kalita, M.C. (2006) Production of thermostable alkaline lipase on vegetable oils from a thermophilic Bacillus sp. DH4, characterization and its potential applications as detergent additive. Journals of Chemistry, Technology and Biotechnology 83, pp. 688–693.,
  6. Borkar, P.S., Bodade, R.G., Rao, S.R. & Khobragade, C.N. (2009) Purification and characterization of extracellular lipase from a new strain-Pseudomonas aeruginosa SRT9. Brazilian Journals of Microbiology 40, pp. 358–366.,
  7. Chartrain, M., Katz, L., Marcin, C., Thien, M., Smith, S., Fisher, E., Goklen, K., Salmon, P., Brix, T., Price, K. & Greasham, R. (1993) Purification and characterization of a novel bioconverting lipase from Pseudomonas aeruginosa MB 5001. Journals of Enzyme Microbial and Technology 15, pp. 575–580.,
  8. Dahiya, P., Arora, P., Chand, S., Chandhury, A. & Dilbaghi, N. (2010) Characterization of an extracellular alkaline lipase from Pseudomonas mondocina M-37. Journal of Basic Microbiology 50, pp. 420–426.,
  9. Damaso, M.C.T., Passianoto, M.A., Freitas, S.C., Freire, D.M.G., Lago, R.C.A. & Couri, S. (2008) Utilization of agro industrial residues for lipase production by solid state fermentation. Brazillian Journals of Microbiology 39, pp. 676–681.,
  10. Erdogan, E.E. & Karaca, A. (2011) Bioremediation of crude oil polluted soil. Asian Journal of Biotechnology 3(3), pp. 206–213.,
  11. Fodiloglu S. & Erkmen O. (1999) Lipase production by Rhizopus oryzae growing on different carbon and nitrogen sources. Journal of Science and Food Agriculture 79, pp. 1936–1938.,
  12. Garlapati, V.K., Vundavilli, P.R. & Banerjee, R. (2010) Evaluation of lipase production by genetic algorithm and particle swarm optimization and their comparative study. Journals of Applied Biochemistry and Biotechnology 162 (5), pp. 1350–1361.,
  13. Gilbert, J.E., Cornish, A. & Jones, C.W. (1991) Purification and properties of extracellular lipase from Pseudomonas aeruginosa EF2. Journal of General Microbiology 137, pp. 2223–2229.,
  14. Gupta, R., Gupta, N. & Rathi, P. (2004) Bacterial lipases: An overview of production, purification and biochemical properties. Journals of Applied Microbiology and Biotechnology 64, pp. 763–781.,
  15. Gupta, R., Rathi, P. & Bradoo, S. (2003) Lipase mediated upgradation of dietary fats and oils. Revision of Food Science and Nutrition 43, pp. 635–644.,
  16. Gupta, N., Shai, V. & Gupta, R. (2014) Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor. Journals of Process Biochemistry 42, pp. 518–526.,
  17. Jinwal, U.K., Roy, U., Chowdhury, A.R., Bhaduri, A.P. & Roy, P.K. (2003) Purification and characterization of an alkaline lipase from a newly isolated Pseudomonas mendocina PK-12CS and chemoselective hydrolysis of fatty acid esters. Bioorganic & Medicinal Chemistry 11 (6), pp. 1041–1046.,
  18. Karigar, S.C. & Rao, S.S. (2011) Role of microbial enzymes in the bioremediation of pollutants: A Review. Enzyme Research, pp. 23–26, doi: 10.4061/2011/605187.,
  19. Khattabi, M., Van Gelder, P., Bitter, W. & Tommassen, J. (2003) Role of the calcium ion and the disulfide bond in the Burkholderia glumae lipase. Journal of Molecular Catalysis B: Enzymatic 22 (5–6), pp. 329–338.,
  20. Kukreja, V. & Bera, M.B. (2005) Lipase from Pseudomonas aeruginosa MTCC 2488: Partial purification, characterization and calcium dependent thermostability. Indian Journal of Biotechnology 4, pp. 222–226.,
  21. Kunamnein, A., Poluri, E. & Davuluri, S. P. (2003) Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE- 11. AAPS Pharmacy and Science Technology 4(4) article 56.,
  22. Lotrakul, P. & Dharmsthiti, S. (1997). Lipase production by Aeromonas sobria LP004 in a medium containing whey and soybean meal. World Journal of Microbiology and Biotechnology 13, pp. 163–166.,
  23. Miller, S., Ross-Inta, C. & Giulivi, C. (2007) Kinetic and proteomic analyses of S-nitrosoglutathione-treated hexokinase A: consequences for cancer energy metabolism. Amino Acids 32 (4), pp. 593–602.,
  24. Mussarat, S., Aamer, A.S., Abdul, H. & Fariha, H. (2008) Influence of culture conditions on production and activity of protease from Bacillus subtilis BS1. Pakistan Journal of Botany 40, pp. 2161–2169.,
  25. OJO, O.O. & AJELE, J.O. (2011) Isolation, purification, characterization and the possible involvement of histidine and cysteine in the catalytic mechanism of beta-amylase sourced from cassava (Manihot esculenta Crantz) peel. Pakistan Journal of Nutrition 10 (9), pp. 823–830.,
  26. Olivera, E., Carnicero, R., Jodra, B., Minambres, B., Garcia, G., Abraham, A., Gallardo, J.S. & Roman, J.L. (2001) Genetically engineered Pseudomonas: A factory of new bioplastics with broad applications. Journals of Environmental Microbiology 10, pp. 612–618.,
  27. Ramani, K., Kennedy, L.J., Ramakrishnan, M. & Sekaran, G. (2010) Purification, characterization and application of acidic lipase from Pseudomonas gessardii using beef tallow as a substrate for fats and oil hydrolysis. Journals of Process and Biochemistry 45, pp. 1683–1691.,
  28. Sarowar, J., Shaela, P., Shariar, S., Sharma, R. & Habibur, R. (2012) Effect of Metal Ions, Chelating Agent and SH Reagents on Radish (Raphanus sativus L.) Root Amylase. Journals of Stress Physiology &Biochemistry 8, pp. 180–188.,
  29. Sebdani, R.M., Ardakani, M.R., Ghezelbash, G.R. & Sadrinasab, M. (2011) Phylogenic characterization of lipase Bacillus strains isolated from Persian gulf sediments. Australian Journal Basic Applied Science 5, pp. 121–126.,
  30. Shah, K.R. & Bhatt, S.A. (2011) Purification and characterization of lipase from Bacillus subtilis Pa2. Journals of Biochemistry Technology 3(3), pp. 292–295.,
  31. Sidhu, P., Sharma, R., Soni, S.K. & Gupta, J.K. (1998) Production of extracellular alkaline lipase by a new thermophilic Bacillus sp. Folia Microbiologica 43(1), pp. 51–54.,
  32. Singh, S.K., Felse, A.P., Nunez, A., Foglia, T.A. & Gross, R.A. (2003) Regioselective enzyme-catalyzed synthesis of sophorolipid esters, amides and multifunctional monomers. Journal of Organic Chemistry 68 (14), pp. 5466–5477.,
  33. Stryer, L., Berg, J.M. & Tymoczko, J.L. (2002) Biochemistry. 5th edition. New York.,
  34. Tan, T. & Yin, C. (2003) The mechanism and kinetic model for glycerolysis by 1, 3 position specific lipase from Rhizopus arrhizus. Biochemistry Engineering Journals 25, pp. 39–45.,
  35. Van der Meer, J., Ravatn, J. & Sentchilo, V. (2001) The element of Pseudomonas sp. strain B13 and other mobile degradative elements employing phage-like integrases. Journal of Microbiology 175, pp. 79–85.,
  36. Verma, N., Thakur, S. & Bhatt, A.K. (2012) Microbial Lipases: Industrial Applications and Properties (A Review). International Research Journals of Biological Science 1 (8), pp. 88–92.,
  37. Yamamato, K. & Fujiwara, N. (1988) Purification and some properties of a castoroil-hydrolyzing lipase from Pseudomonas sp. Journal of Agricultural Biology and Chemistry 52, pp. 3015–3021.,
  38. Yuzo, T. Kojima, N. & Sakaya, R. (2003) Purification and characterization of the lipase from Pseudomonas fluorescens HU 380. Journal of Bioscience and Bioengineering 96 (3), pp. 211–226.,

Title

Kinetic properties of Lipase obtained from Pseudomonas aeruginosa isolated from crude oil contaminated soil

Pages

154-161

Figures

9

Tables

1

ISSN printed

1733-8670

ISSN on-line

2392-0378

DOI

10.17402/363

Type

Journal

59 Scientific Journals of the Maritime University of Szczecin, no. 59 / 2019

Publisher

Scientific Journals Maritime University of Szczecin, Zeszyty Naukowe Akademia Morska w Szczecinie

Copyright Holder

Scientific Journals Maritime University of Szczecin, Zeszyty Naukowe Akademia Morska w Szczecinie

Published

2019-10-04

Recieved

2019-04-04

Accepted

2019-07-29

Funding

No data

Keywords

biodegradation, enzymes, gel filtration, lipase, olive oil, Pseudomonas aeruginosa

URI:

https://repository.am.szczecin.pl/handle/123456789/2542

Language

English

Authors

Femi-Ola, Titilayo Olufunke1; Orjiakor, Paul Ikechukwu2; Enerijiofi, Kingsley Erhons3; Oke, Iyanuoluwa Omolade4; Fatoyinbo, Adebayo Adeola5

Affiliation

1 Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria,
2 Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria,
3 Samuel Adegboyega University, College of Basic and Applied Sciences Department of Biological Sciences, Ogwa, Edo State, Nigeria, paulorjiakor@gmail.com
4 Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria,
5 Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria,

Show full item record

Files in this item

Download paper in PDF format
Name: 18-zn-am-59-131-f...
Size: 614.03 KB
Format: PDF
Licence: CC BY 4.0
Download

This item appears in the following Collection(s)

Search repository

Advanced Search

Browse

My Account

RSS Feeds

XML

Cite